Table 1.
Sensitivity of RDT-DNA extraction methods in in vitro cultured parasites
| RDT Fragment | Simple elution | Chelex-100 | ABI |
|---|---|---|---|
| Paracheck-Pf | |||
| 1 cm |
§ |
2 |
200 |
| Proximal |
NA |
20 |
200 |
| Distal |
NA |
NA |
20 |
| Whole |
NA |
NA |
20 |
| SD-Bioline Malaria P.f/Pan | |||
| 1 cm |
2 |
2 |
20 |
| Proximal |
NA |
2 |
20 |
| Distal |
NA |
NA |
20 |
| Whole |
NA |
NA |
20 |
| Filter paper | |||
| 5 μL blood spot | 200 | 2 | 200 |
Lowest achieved parasite detection levels (parasites/μL) for DNA extraction from Paracheck-Pf, SD-Bioline Malaria P.f/Pan and from 5 μL blood spotted on filter paper.
§ = Not estimated due to negative results.
NA = Not applicable, the size of the RDT fragment used for extraction was limited by the extraction volume.