Table 1.
Vectors used for restriction enzyme mediated selectable marker gene disruption
| Original Vector (and source) | Phenotype of original selectable marker | Cloning restriction site | Phenotype of cloned selectable marker | Construct name |
|---|---|---|---|---|
| pUC19 (New England Biolabs) |
ampR |
ScaI |
kanR |
pUC19:kanR |
| pUC19 (New England Biolabs) |
ampR |
ScaI |
tetR |
pUC19:tetA |
| pZErO-2 (Invitrogen) |
kanR |
NcoI |
tetR |
pZErO-2:tetA |
| pBlueScript SK + (Stratagene) |
ampR |
ScaI |
kanR |
pBlueScript SK+: kanR |
| pET23a (Novagen) | ampR | PstI | tetR | pET23a:tetA |
ampR : Ampicillin resistance, kanR : Kanamycin resistance, tetR : Tetracycline resistance.