Table 5.

Commonly used vectors and unique restriction sites suitable for selectable marker restriction enzyme mediated gene disruption

Vector	Selectable marker	Source	Unique restriction sites in selectable marker
pUC18, pUC19	ampR	New England Biolabs	XmnI, ScaI
pBlueScript	ampR	Stratagene	ScaI
pZErO	kanR	Invitrogen	NcoI
pBR322	ampR tetR	New England Biolabs	ScaI, PvuI, PstI EcoRV, BamHI, SphI, SalI
pACYC177	ampR kanR	New England Biolabs	ScaI, PstI, BglI ClaI, HindIII
pACYC184	camR tetR	New England Biolabs	EcoRI, NcoI EcoRV, BamHI, SphI, SalI

amp^R : ampicillin resistance, kan^R : kanamycin resistance, tet^R : tetracycline resistance, cam^R : chloramphenicol resistance.