Figure 7. CLSM localization of V-ATPase (green) and cathepsin K (red) in osteoclasts.

A–C: Osteoclast incubated on bone shows strong immunolocalizion of V-ATPase in which a patch of cathepsin K immunolocalization is also seen, in a region free of V-ATPase. D: an osteoclast incubated on glass showing cathepsin K immunolocalized in the center of this well-spread cell, while V-ATPase is observed in small patches at the periphery, immediately adjacent to the actin ring (blue). C and D are also counterstained with DAPI to show nuclei. Bar 10 µm.