Table 1.
Purification of the recombinant BglMKg (U for β-galactosidase activity)
Step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
---|---|---|---|---|---|
Crude extract |
434.5 |
759 |
1.75 |
1.0 |
100 |
Fractogel EMD DEAE | 72.0 | 302 | 4.19 | 2.4 | 40 |