Table 4.
Substrate | Temperature (°C) | Km (mM) | kcat (s-1) | kcat/Km (s-1mM-1) |
---|---|---|---|---|
|
10 |
4.82 ± 1.10 |
3.99 ± 0.53 |
0.83 |
ONPGal |
20 |
9.04 ± 1.39 |
12.43 ± 1.35 |
1.37 |
30 |
5.72 ± 0.60 |
14.73 ± 0.95 |
2.57 |
|
|
10 |
0.13 ± 0.06 |
4.46 ± 0.28 |
34.31 |
PNPGlc |
20 |
0.16 ± 0.03 |
11.74 ± 0.26 |
73.37 |
30 |
0.37 ± 0.04 |
22.45 ± 0.64 |
60.67 |
|
|
10 |
73.89 ± 46.97 |
7.95 ± 3.99 |
0.11 |
Lactose |
20 |
41.56 ±13.31 |
8.55 ±1.89 |
0.12 |
30 |
86.91 ± 20.32 |
24.30 ±4.64 |
0.28 |
|
|
10 |
3.55 ± 1.76 |
6.35 ± 0.7 |
1.79 |
Cellobiose | 20 |
3.89 ± 0.98 |
10.46 ± 0.67 |
2.72 |
30 | 2.76 ± 0.51 | 11.19 ± 0.43 | 4.05 |