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. 2013 Mar 15;13:22. doi: 10.1186/1472-6750-13-22

Table 4.

Kinetic parameters of BglMKg

Substrate Temperature (°C) Km (mM) kcat (s-1) kcat/Km (s-1mM-1)
 
10
4.82 ± 1.10
3.99 ± 0.53
0.83
ONPGal
20
9.04 ± 1.39
12.43 ± 1.35
1.37
30
5.72 ± 0.60
14.73 ± 0.95
2.57
 
10
0.13 ± 0.06
4.46 ± 0.28
34.31
PNPGlc
20
0.16 ± 0.03
11.74 ± 0.26
73.37
30
0.37 ± 0.04
22.45 ± 0.64
60.67
 
10
73.89 ± 46.97
7.95 ± 3.99
0.11
Lactose
20
41.56 ±13.31
8.55 ±1.89
0.12
30
86.91 ± 20.32
24.30 ±4.64
0.28
 
10
3.55 ± 1.76
6.35 ± 0.7
1.79
Cellobiose 20
3.89 ± 0.98
10.46 ± 0.67
2.72
30 2.76 ± 0.51 11.19 ± 0.43 4.05