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. 2013 Mar 21;8(3):e59512. doi: 10.1371/journal.pone.0059512

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© 2013 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Human cord blood-derived CD34⁺ cells were treated with LT on day 12 and then analyzed on day 16 in aforementioned 16-day differentiation courses of megakaryocyte. The cell size (FSC) and cell granularity (SSC) are indicated (A). Size (FSC) and granularity (SSC) parameters of cord-blood platelets were plotted as linear scale (b-1) and logarithmic scale (b-2). The cellular DNA contents were revealed by propidium iodine (PI) staining (b-3). Agonist ADP, thrombin, and collagen-induced stimulation of the platelets (b-4) versus cell sorter-enriched R2-cells (day 16, LT-treated groups) (C) was revealed by enhanced surface staining of P-selectin (CD62P), a platelet activation marker. These results obtained from 3 independent experiments.