Skip to main content
. 2013 Mar 21;8(3):e60271. doi: 10.1371/journal.pone.0060271

Figure 2. Multimodal imaging was used to obtain in vivo AA diameter measurements in stage 21 chick embryos.

Figure 2

Video recordings from fluorescent dye microinjections were processed to obtain individual AA identity and midpoint diameter data (A,B). OCT was used to obtain transverse (C,D) and longitudinal (E,F) sections through the AA to analyze midpoint diameter and AA tapering. Both the right (A,C,E) and left (B,D,F) laterals were imaged. In D, the dotted circles represent the AA lumen based on our image processing algorithm. The dotted lines in F highlight the boundaries of the AA lumen. Comparison between imaging modalities showed good agreement (p>0.05). The axes in C-F demonstrate the dorsal (dors), ventral (ven), cranial (cra), caudal (cau), right (R), and left (L) directions to orient the reader. All scale bars are 500 µm.