Table 2.
Primers for real-time quantitative PCR
| Gene | Accession number | 5′-3′sequence | Amplicon size |
|---|---|---|---|
| IGF1 |
NM_001077828 |
Fw: AGTTGGTGGATGCTCTCCAGT |
115 |
| |
|
Rw: CACTCATCCACGATTCCTGTC |
|
| IGF1R |
NM_001244612 |
Fw: GGACGCAGTACGCCGTTTAC |
187 |
| |
|
Rw: AGGGAGGGCGGGTTCCACTT |
|
| PPP3CA |
NM_174787 |
Fw: CAAGGCAATTGATCCCAAGT |
204 |
| |
|
Rw: AGAATTGAAGCCCCCTCTGT |
|
| NFATc1 |
NM_001166615 |
Fw: TACGAGCTGAGGATCGAGGT |
139 |
| |
|
Rw: GAGGCTCGCTCTCCACATAG |
|
| MEF2C |
NM_001046113 |
Fw: CAGTCATTGGCTACCCCAGT |
151 |
| |
|
Rw: GCGGTGTTAAAGCCAGAGAG |
|
| GSK3b |
NM_001101310 |
Fw: TTCCTTTGGAATCTGCCATC |
233 |
| |
|
Rw: ACAGCTCAGCCAACACACAG |
|
| TEAD1 |
XM_002693050 |
Fw: CTCAGGACAGGGAAGACGAG |
134 |
| |
|
Rw: GGCTGCCCTGTTTGTATCAT |
|
| MSTN |
NM_001001525 |
Fw: GTGTTGCAGAACTGGCTCAA |
256 |
| Rw: CAGCATCGAGATTCTGTGGA |
Primer sequences used for real-time quantitative PCR were designed on Bos taurus GenBank mRNA sequences using Primer 3 Software (version 4.0). Table summarizes primer information including sequences, gene accession number and amplicon sizes.