Figure 4. Inhibition of intracellular signaling pathways.

NK cells from six controls were cultured with K562 and PMA-I alone (control) or in the presence of the inhibitor drugs LY294002 (25 µM), PD98059 (25 µM), Rapamycin (100 nM) and Rottlerin (5 µM). NK cell function was assessed for CD107a expression (A) and IFN-γ production (B) in response to addition of inhibitor drugs in culture. CD107a expression is shown to be reduced by addition of inhibitor drugs affecting pathways of cellular activation. Statistical significance was defined as p<0.05. Graphed data presented as mean ± SEM.