Figure 8. Cell lysis of the S. japonicus ura4 deletion mutant in the presence of polypeptone.

(A) Serially diluted cells (10-fold) of S. pombe L972 (WT) and UMP31 (Δura4), and S. japonicus NIG2028(h⁻) and NIG5091(h⁻ ura4-D3) strains were grown on the indicated plates and incubated for 3 days at 30°C. For the alkaline phosphatase assay, BCIP was used as described in Fig. 1. (B) Cellular morphologies of the indicated strains after being grown on YPD plates for 3 days at 30°C. Scale bar: 10 µm.