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(A) Representation of the M. tuberculosis genomic region (2408385–2424838), showing ORFs and gaps, and highlighting regions upstream of the dcw operon screened for the presence of a promoter driving the operon. (B) cDNA analysis for identifying boundaries of the dcw operon. (C) Promoter analysis by cloning into the promoter-less vector pYUB76 and β-galactosidase assay for confirmation of promoter activity.
