Table 1. Kinetic parameters of MurC, MurD and MurF proteins for endogenous substrates.
MurC | MurD | MurE¤ | MurF | |
K M ATP (µM) | 67.7±1.1 | 106.4±0.1 | 120 | 92.3±0.2 |
K M UNAM/UMA/UMAG/UMT * (µM) | 23.5±0.5 | 53.3±0.1 | 40 | 64.5±0.2 |
K M L-Ala/D-Glu/mDAP/D-Ala-D-Ala (µM) | 43.0±0.9 | 85.1±0.2 | 69 | 78.1±0.2 |
V max (µmol min−1mg−1) | 1.2±0.1 | 0.8±0.1 | 1.2 | 0.9±0.01 |
k cat (sec−1) | 1.0±0.01 | 0.8±0.2 | 1.2 | 1.4±0.5 |
k cat/K M ATP (mM) | 15.1±0.6 | 7.5±0.1 | 10 | 15.3±0.1 |
k cat/K M UNAM/UMA/UMAG/UMT (sec−1 mM−1) | 44.8±0.3 | 15.1±0.1 | 30 | 21.7±0.2 |
k cat/K M L-Ala/D-Glu/mDAP/D-Ala-D-Ala (sec−1 mM−1) | 23.9±0.23 | 9.4±0.12 | 17.4 | 18.1±0.3 |
k cat (E. coli) (sec−1) | 15.4# | 15.5§ | - | 13.1¥ |
K M L-Ser (µM) | 99.7±0.5 | |||
K M Gly (µM) | 146.5±1.6 | |||
k cat L-Ser (sec−1) | 1.2±0.01 | |||
k cat Gly (sec−1) | 1.53±0.1 | |||
k cat/K M L-Ser (sec−1 mM−1) | 12.5±0.02 | |||
k cat/K M Gly (sec−1 mM−1) | 10.47±0.1 |