Table 1. Kinetic parameters of MurC, MurD and MurF proteins for endogenous substrates.

	MurC	MurD	MurE¤	MurF
K M ATP (µM)	67.7±1.1	106.4±0.1	120	92.3±0.2
K M UNAM/UMA/UMAG/UMT * (µM)	23.5±0.5	53.3±0.1	40	64.5±0.2
K M L-Ala/D-Glu/mDAP/D-Ala-D-Ala (µM)	43.0±0.9	85.1±0.2	69	78.1±0.2
V max (µmol min−1mg−1)	1.2±0.1	0.8±0.1	1.2	0.9±0.01
k cat (sec−1)	1.0±0.01	0.8±0.2	1.2	1.4±0.5
k cat/K M ATP (mM)	15.1±0.6	7.5±0.1	10	15.3±0.1
k cat/K M UNAM/UMA/UMAG/UMT (sec−1 mM−1)	44.8±0.3	15.1±0.1	30	21.7±0.2
k cat/K M L-Ala/D-Glu/mDAP/D-Ala-D-Ala (sec−1 mM−1)	23.9±0.23	9.4±0.12	17.4	18.1±0.3
k cat (E. coli) (sec−1)	15.4#	15.5§	-	13.1¥
K M L-Ser (µM)	99.7±0.5
K M Gly (µM)	146.5±1.6
k cat L-Ser (sec−1)	1.2±0.01
k cat Gly (sec−1)	1.53±0.1
k cat/K M L-Ser (sec−1 mM−1)	12.5±0.02
k cat/K M Gly (sec−1 mM−1)	10.47±0.1

^*UNAM: UDP-MurNAc; UMA: UDP-MurNAc-L-Ala; UMAG: UDP-MurNAc-L-Ala-D-Glu; UMT: UDP-MurNAc-L-Ala-γ-D-Glu-m-DAP.

^¤ [15].

^# [27].

^§ [67].

^¥ [32].