Figure 5. IFN-γ/IL-17 enhance the antimicrobial activity of primary keratinocytes.

Primary keratinocytes were stimulated with IL-17A (40 ng/ml), IFN-γ (40 ng/ml) or IFN-γ/IL-17A (each 20 ng/ml) for 24 h. Cell lysates were incubated with Staphylococcus aureus and bacteria growth was determined by measuring optical density at 600 nm (OD₆₀₀). Data are derived from three experiments each done in triplicates and statistical differences were analyzed using one-way ANOVA with Tukey’s test (*p<0.05, ns = not significant).