Skip to main content
. 2013 Mar 21;8(3):e59965. doi: 10.1371/journal.pone.0059965

Figure 3. Role of nmMLCK in thrombin-induced RelA/p65 nuclear translocation and phosphorylation.

Figure 3

( A ) Effect of inhibition of nmMLCK on thrombin-induced nuclear translocation of RelA/p65. EC were pretreated with ML-7 (10 µM) and then challenged with thrombin (5 U/ml) for 1 h. Nuclear extracts were separated by SDS-PAGE and immunoblotted with anti-RelA/p65 antibody. TATA-binding protein (TBP) levels were used to monitor loading. The bar graph represents the effect of nmMLCK inhibition on thrombin-induced RelA/p65 nuclear translocation. Data are means±S.E. (n = 3–4 for each condition). **, p<0.01 compared with untreated control; #, p<0.05 compared with thrombin-stimulated control. ( B ) Effect of inhibition of nmMLCK on thrombin-induced phosphorylation of RelA/p65. EC were pretreated with ML-7 (10 µM) and then challenged with thrombin (5 U/ml) for 1 h. Total cell lysates were immunoblotted with an anti-phospho-RelA/p65 (Ser536) antibody. RelA/p65 levels were used to monitor loading. The bar graph represents the effect of nmMLCK inhibition on thrombin-induced RelA/p65 phosphorylation. Data are means±S.E. (n = 3 for each condition). **, p<0.01 compared with untreated control; ##, p<0.01 compared with thrombin-stimulated control.