Figure 3. Differences in tumor regression in S180-cancer susceptible C57BL/6 mice after the AT of leukocytes from either cancer resistant SR/CR mice or non-resistant controls.
(A) Survival of S180-cancer susceptible C57BL/6 mice after AT. Two mice that received SR/CR-leukocytes and four mice that received C57BL/6 leukocytes were euthanized due to excessive S180 tumor burdens. (B) Tumor volumes after AT. Progressive tumor growth was observed in untreated C57BL/6 mice after the bilateral s.c. injection of 106 S180 cells in the axillary region. The growth reached a plateau approximately 20 days post tumor cell inoculation. One mouse was euthanized when one of its tumors exceeded 12 mm in diameter. The vertical line at 40 days represents the time point when mice with completely regressed tumors were injected i.p. with S180 cells. A one-way ANOVA analysis revealed a significant difference (p = 0.0005) in tumor size between the two groups of mice with either efficient or inefficient AT. Each point represents the mean and SEM. (C) The numbers and the composition of the transferred SR/CR and C57BL/6 (B6) derived leukocytes. There were no significant differences in the numbers of total leukocytes (splenocytes + peritoneal leukocytes) transferred from the SR/CR and C57BL/6 mice (p = 0.969). The numbers of peritoneal immune cells derived from the SR/CR and C57BL/6 mice were not significantly different (p = 0.721). T = total leukocytes. Per = peritoneal leukocytes.