Figure 1. Example of histology images and morphometric image analysis.

A) Histologic image of a liver (Masson trichrome, ×20 objective), B) Binary image of same image after application of threshold, C) Mask showing fat vacuoles after application of size and structural criteria, D) Examples of binary histology images with measured fat percentage areas. These images have been thresholded as in 1B, but not masked (as in 1C), so as to keep the additional white spaces that are not represented in the areal fat estimate, but are visible in a histology image. Each square is 500 microns across.