Fig 2.

Characterisation of protease activity present in 48 h A. fumigatus cultures containing different protein substrates. (A) Rate of protease activity was determined in culture supernatant from 48 h cultures of A. fumigatus. Protease activity was normalised against dry biomass to account for differences in growth rate. (B) Inhibition of protease activity in culture supernatant from 48 h cultures of A. fumigatus. Percentage change in activity with inhibitor was relative to uninhibited control supernatant set at 100 % for each culture. Data represents means ± SD and was analysed by t-test, compared to Vogel's medium (n = 3 biological repeats).