Fig 3.

Identification of proteases secreted by A. fumigatus in response to different protein substrates. (A) Equal volumes of filtered and dialysed culture supernatant was subjected to size exclusion chromatography and protein levels measured continuously by UV absorbance at 280 nm. (B) Fractions collected at 1 ml intervals were analysed for protease activity. (C) SDS-PAGE of proteolytically active fractions showing bands analysed for protein identification by LC-MS/MS (see Table 1).