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Protease gene expression in A. fumigatus cultures in response to different protein substrates. Protease gene expression was assayed by qPCR and expression of Mep, Alp 1, and Lap 1 was normalised against β-tubulin. Differences in gene expression in protein containing media compared with control Vogel's minimal medium were calculated using 2ΔΔct. Data represents means ± SD (n = 3 biological repeats) and was anaylsed by both one- and two-way ANOVA.
