Table 1.

Proteins identified by LC-MS/MS in A. fumigatus culture supernatants. The SDS-PAGE bands are shown in Fig 3C and the number of peptide matches for each protein is shown. A confident match was considered to be any protein with three or more matched peptides. Proteases are highlighted in bold. Protein molecular weights are quoted from UniProt for protein translated from the full ORF.

Identified proteins	Molecular weight (kDa)	CADRE accession number	UniProt accession number	Protein matches (band and number of matched peptides)
Casein medium	Pig lung medium	Mucin medium
Exo- β −1,3-glucanase	84	AFUA_6G13270	Q4WLJ9	–	L (3)	–
β-d-glucoside glucohydrolase	78	AFUA_7G06140	Q4WGT3	A (18)	L (12)	T (5) V (3)
Aminopeptidase Y (Lap 1)	54	AFUA_3G00650	Q5VJG5	B (13) C (6) D (5)	–	–
Mannosidase MsdS	54	AFUA_1G14560	Q6PWQ1	C (14)	M (14)	U (8)
FAD-dependent oxygenase	55	AFUA_3G00840	Q4WFW0	C (11)	M (9)	U (7)
Oxidoreductase, FAD-binding	50	AFUA_2G14480	Q4X072	C (4)	M (4)	–
Uncharacterised protein	49	AFUA_4G03200	Q4W9Z5	D (12)	N (10)	–
Glucooligosaccharide oxidase	51	AFUA_6G14340	Q4WL94	D (5)	–	–
Elastinolytic metalloproteinase (Mep/Asp f 5)	69	AFUA_8G07080	P46075	E (5)	N (5)	–
Cell wall β-1,3-endoglucanase	45	AFUA_3G00270	Q4WG16	F (7)	O (4)	V (4)
Class V chitinase	46	AFUA_3G07160	A4D9F7	F (3)	N (6)	–
Uncharacterised protein	37	AFUA_5G01120	Q4WDY6	F (4)	–	–
Uncharacterised protein	37	AFUA_8G00630	Q4WB08	–	P (5)	–
Alkaline serine protease (Alp1/Asp f 13)	42	AFUA_4G11800	P28296	G (8) H (4)	P (6) Q (3) S (5)	V (5) W (5) X (3) Y (3)
Chitosanase	25	AFUA_4G01290	Q875I9	H (9)	–	–
Uncharacterised protein	33	AFUA_5G10930	Q4WV60	H (4)	–	–
Cell wall protein PhiA	19	AFUA_3G03060	Q4WF87	I (3) J (3)	–	–
Cell wall protein	19	AFUA_3G01130	Q4WFT1	K (12)	R (8)	X (9)
Cu/Zn Superoxide Dismutase	16	AFUA_5G09240	Q9Y8D9	K (4)	–	–
Allergen Asp f 15	16	AFUA_2G12630	O60022	–	–	X (3)
Secreted antimicrobial peptide	10	AFUA_8G00710	Q4WB16	–	–	Y (3)