Figure 3.

mir-92a Represses Trichome Development on the T2 Femur

Uniform expression of mir-92a represses trichome formation progressively depending on developmental timing of overexpression induced by heat shock. Tan shading indicates the extent of trichome-free cuticle. See also Figure S3.

(A–D) Posterior of T2 femurs of F1 flies from the cross between HS-GAL4 and UAS-mir-92a.

(A) Posterior T2 femur of a control F1 fly that was not heat shocked.

(B–D) F1 flies heat shocked at 8 hr (B), 16 hr (C), and 24 hr (D) after puparium formation (APF).

(E) Posterior T2 femur of the dac-GAL4 control line.

(F) UAS-mir-92a expression driven by dac-GAL4 represses trichome formation throughout the posterior femur.

(G) UAS-shaΔ3UTR driven by dac-GAL4 results in the development of ectopic trichomes and removes the naked valley.

(H) Simultaneous overexpression of UAS-shaΔ3UTR and UAS-mir-92a using dac-GAL4 leads to rescue of trichome formation and removes the naked valley.