Table 1.
Summary of PCR primers used in gene expression analysis.
| Gene | Encoding | Primer sequence |
|---|---|---|
| (F)orward (5′–3′) | ||
| (R)everse (5′–3′) | ||
| ACTB | Beta actin (β-actin) | (F)CCAACCGCGAGAAGATGA |
| (R)CCAGAGGCGTACAGGGATAG | ||
| AMPH2 | Bridging integrator 1 (BIN1) | (F)ACGGGAGCAACACCTTCA |
| (R)GCCGCGAAAACAGTTTACTT | ||
| ATP2A2 | Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2) | (F)AACGTCGGGGAAGTTGTCT |
| (R)GAATCAAAGCCTCGGGAAAT | ||
| CACNA1C | L-type voltage-dependent Ca2+ channel (Cav1.2), alpha 1C subunit | (F)TGACATCGAGGGAGAAAACT |
| (R)ACATTAGACTTGACTGCGGC | ||
| CACNA1G | T-type voltage-dependent Ca2+ channel (Cav3.1), alpha 1G subunit | (F)TGCTCTTCAATTTGCTGGTC |
| (R)TCTTCCCGTTTGCTGATTTC | ||
| CALR | Calreticulin | (F)CTATGATAACTTTGGCGTGCTG |
| (R)CTCCTCAGCGTATGCCTCAT | ||
| CASQ2 | Calsequestrin 2 | (F)GAGTTTGATGGCGAGTTTGC |
| (R)TTGCTGCTGATGATCTCCAC | ||
| CAV3 | Caveolin 3 | (F)GAGGCCCAGATCGTCAAG |
| (R)TCACGTCTTCAAAATCCACCT | ||
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | (F)GTC AGT GGT GGA CCT GAC CT |
| (R)CCC TGT TGC TGT AGC CAA AT | ||
| GATA4 | GATA binding protein 4 (GATA4) | (F)GGAAGCCCAAGAACCTGAAT |
| (R)GTTGCTGGAGTTGCTGGAA | ||
| ITPR2 | Inositol 1,4,5-trisphosphate receptor, type 2 (IP3R) | (F)CCTACTCCAAAACTGCACAGG |
| (R)GTCTGACATTGATATCCCCATCT | ||
| JPH2 | Junctophilin 2 | (F)AACATTGCTCGCACTTTGG |
| (R)GCTTCTGATATTCCGGACCTG | ||
| MYH6 | Myosin heavy chain 6 (α-MHC) | (F)CTCAAGCTCATGGCCACTCT |
| (R)GCCTCCTTTGCTTTTACCACT | ||
| MYH7 | Myosin heavy chain 7 (β-MHC) | (F)ACACCCTGACTAAGGCCAAA |
| (R)TCCAGGGATCCTTCCAGAT | ||
| MYL2 | Ventricular myosin light chain 2 (MLC2v) | (F)ACA TCA TCA CCC ACG GAG AAG AGA |
| (R)ATT GGA ACA TGG CCT CTG GAT GGA | ||
| NKX2-5 | Homeobox protein Nkx-2.5 (Nkx-2.5) | (F)ACC TCA ACA GCT CCC TGA CTC T |
| (R)ATA ATC GCC GCC ACA AAC TCT CC | ||
| NPPA | Natriuretic peptide A (ANF) | (F)CAGGATGGACAGGATTGGAG |
| (R)TCCTCCCTGGCTGTTATCTTC | ||
| PLN | Phospholamban | (F)TGATGATCACAGCTGCCAAG |
| (R)TGAGCGAGTGAGGTATTGGA | ||
| PPIG | Peptidylprolyl isomerase G (Cyclophilin G) | (F)CTTGTCAATGGCCAACAGAGG |
| (R)GCCCATCTAAATGAGGAGTTGGT | ||
| RN18S | 18S ribosomal RNA (18S) | (F)GCAATTATTCCCCATGAACG |
| (R)GGGACTTAATCAACGCAAGC | ||
| RYR2 | Ryanodine receptor 2 (RyR) | (F)CTGCGCCATTCCTATAGTGG |
| (R)AGTTGAAGACCGGGAGGTG | ||
| SLC8A1 | Solute carrier family 8 (Na+/Ca2+ exchanger), member 1 (NCX) | (F)GGTTGGGACTAACAGCTGGA |
| (R)CCACATTCATCGTCGTCATC | ||
| TCAP | Titin-cap (Telethonin) | (F)GGCAGAATGGAAGGATCTGA |
| (R) TCTCATGTCTCTGGGTGTCCT | ||
| TNNT2 | Troponin T type 2 (Cardiac) (cTnT) | (F)TTC ACC AAA GAT CTG CTC CTC GCT |
| (R)TTA TTA CTG GTG TGG AGT GGG TGT GG | ||
| TRDN | Triadin | (F)ACATATTTGTCCATGGGGATTT |
| (R)TGGAAGCTTGTTCTGTCGGTA |