Fig. 3.

Chemotactic response of donor cells from uninfected pigs to cell supernatants from macrophages either mock infected or infected with ASFV isolates Benin 97/1 or OURT88/3. Blood derived macrophages were infected with ASFV isolates Benin 97/1 or OURT88/3 or mock-infected. At 16 h post-infection cell supernatants were harvested and the chemotactic substances present were measured by (A) migration of PBLs from uninfected donor pigs using a 96-well neuroprobe assay or (B) by measuring by flow cytometry the shape change induced in PMN cells from uninfected donor pigs. In panel A the chemotactic index is shown on the x axis and the response of donor cells from 3 uninfected pigs (labelled 1, 2, 3) to supernatants from infected cells from 5 different pigs is indicated on the x axis. Panel B shows the change in forward scatter of PMN cells on the y axis as the percentage difference compared to control cells measured at 30 min after exposure to supernatants from infected or mock-infected cells. The x axis indicates measurements of PMNs from different uninfected pigs (2, 3, 7, 8). Responses to supernatants from cells from 5 different pigs infected with OURT88/3 are shown in white boxes, to Benin 97/1 in black boxes and mock-infected cells in grey boxes. The mean and standard errors are shown. **Significant difference between groups (p = 0.002). *Significant difference between groups (p = 0.012).