Table 1.
Summary of the densities and Dlat values (lateral diffusion coefficient) of M2 receptors on CHOM2, HL-1, primary cardiomyocytes and cardiac tissue slices.
| CHOM2 | HL-1 | Myocytes | Heart slice | aCHOM1 | |
|---|---|---|---|---|---|
|
Density (μm− 2) (Ncells) |
3.1 ± 0.8b (n = 16) |
7.5 ± 2.8 (n = 22) |
1.8 ± 0.4 (n = 6) |
0.88 ± 0.22c (n = 14) |
2.1 ± 0.02 (n = 9) |
|
Dlat(μm2·s− 1) (Nobjects; Ncells) |
0.109 ± 0.015 (13613; 13) |
0.126 ± 0.013 (27809; 10) |
0.165 ± 0.013 (2712; 6) |
0.63 ± 0.01 (3723; 14) |
0.097 ± 0.006 (21954; 9) |
a
From Hern et al., 2010 (7).
b
Values stated in bold are mean ± SD values in italics indicate the sample size.
c
These values are measured from the observed spots from slices of mouse hearts (5 and 2 days before birth). It was not possible to correct these values for the effect of photobleaching because the cell footprint is very small and molecules move fast into and out of the evanescent field. It is likely that the original density has been underestimated considerably, possibly by a factor of 2–3.