TABLE 2.
Expression changes induced by an HSD in wild-type larval fat body
Wild-type Vw1118; UAS-Dcr2; cgGAL4 larvae were reared on a 0.15 m (control (ctl)) or 0.7 m HSD, and FBs were isolated for RNA extraction. A two-tailed Student's t test was used to derive p values. For simplicity, only a selection of genes is shown here; for full data, please see GEO accession number GSE43734. TCA, tricarboxylic acid; PEPCK, phosphoenolpyruvate carboxykinase.
| CG number/category | Name | Fold change HSD/ctl | p vs. ctl |
|---|---|---|---|
| Trehalose | |||
| CG4104 | Tps1 | 2.23 | 0.040 |
| CG30035 | Tret-1 | 1.72 | 0.018 |
| CG8234 | Tret-2 | 1.89 | 0.026 |
| Glycolysis | |||
| CG8251 | Pgi | 2.10 | 0.003 |
| CG6058 | Aldolase | 1.72 | 0.005 |
| CG1721 | Pglym78 | 1.84 | 0.013 |
| CG17654 | Eno | 1.95 | 0.007 |
| CG7070 | PyK | 1.76 | 0.008 |
| CG9042 | Gpdh | 5.72 | 0.000 |
| Lipogenesis | |||
| CG11198 | ACC | 0.54 | 0.197 |
| CG3523 | Fatty-acid synthase | 1.88 | 0.012 |
| CG17374 | Fatty-acid synthase | 0.51 | 0.152 |
| CG6178 | Fatty-acid-CoA ligase | 3.64 | 0.023 |
| CG3209 | GPAT | 1.32 | 0.028 |
| CG4729 | GPAT | 1.41 | 0.021 |
| CG17608 | GPAT | 1.92 | 0.047 |
| CG31991 | mdy (DGAT) | 1.72 | 0.034 |
| CG8522 | SREBP | 0.88 | 0.078 |
| CG18362 | Mio/dChREBP | 1.71 | 0.000 |
| CG5887 | desat1 | 2.90 | 0.001 |
| Lipolysis | |||
| CG11325 | AKHR | 2.64 | 0.001 |
| CG5560 | Doppelgänger of brummer | 0.63 | 0.001 |
| β-Oxidation | |||
| CG12891 | CPT1 | 1.50 | 0.018 |
| CG2107 | CPT2 | 1.25 | 0.091 |
| CG4630 | Carnitine acyl-carnitine translocase | 2.63 | 0.008 |
| CG6178 | Acyl-CoA synthase | 3.64 | 0.023 |
| CG9527 | Acyl-CoA dehydrogenase | 1.88 | 0.002 |
| CG17544 | Acyl-CoA dehydrogenase | 0.24 | 0.040 |
| CG9547 | Acyl-CoA dehydrogenase | 0.61 | 0.018 |
| CG9709 | Acyl-CoA oxidase 57D-d | 3.09 | 0.073 |
| CG9707 | Acyl-CoA oxidase 57D-p | 0.73 | 0.031 |
| CG8778 | Enoyl-CoA hydratase | 0.77 | 0.040 |
| CG5044 | 3-Hydroxyisobutyryl-CoA hydrolase | 0.76 | 0.002 |
| CG14630 | Carnitine biosynthesis; γ-butyrobetaine dioxygenase activity | 0.76 | 0.027 |
| TCA cycle | |||
| CG9244 | Aconitase | 1.51 | 0.001 |
| CG7998 | Malate dehydrogenase | 1.47 | 0.027 |
| CG7430 | α-Ketoglutarate dehydrogenase | 1.57 | 0.006 |
| Ketone and CoA metabolism | |||
| CG1140 | SCOT (acetoacyl-CoA synthase) | 1.30 | 0.557 |
| CG5725 | fbl (pantothenate kinase) | 1.82 | 0.036 |
| CG10932 | Acetoacyl-CoA thiolase | 1.11 | 0.533 |
| CG9149 | Acetoacyl-CoA thiolase | 1.04 | 0.898 |
| CG10399 | HMG-CoA lyase | 1.79 | 0.456 |
| CG4311 | HMG-CoA synthase | 1.39 | 0.062 |
| CG8322 | ATP-citrate lyase | 1.11 | 0.570 |
| CG1774 | Acyl-CoA thioester hydrolase | 10.59 | 0.000 |
| Gluconeogenesis | |||
| CG17725 | PEPCK | 2.32 | 0.027 |
| CG10924 | PEPCK | 1.75 | 0.024 |
| CG31692 | fbp | 1.56 | 0.060 |
| CG1516 | Pyruvate carboxylase | 2.63 | 0.019 |
| Glycogenolysis | |||
| CG11325 | AKHR | 2.64 | 0.001 |
| CG7254 | GlyP | 1.81 | 0.003 |