FIGURE 2.

Motor neuron differentiation of SBMA-iPSCs and AR expression in differentiated neurons. A and B, expression of the motor neuron progenitor marker islet-1 (A, green) and GFP under the control of the HB9 promoter for the mature neuron marker (green) in βIII-tubulin-positive (βIII-Tub, red) cells (B). Scale bar, 100 μm. C and D, differentiated neurons were double-stained with anti-MAP2 and anti-AR antibodies. ARs are localized primarily in the nuclei. Scale bar in C, 200 μm. Scale bar in D, 20 μm.