FIGURE 4.

LSTc decoy liposomes extend survival after lethal IAV challenge. 1000 PFU of PR/8 was incubated with control liposomes or LSTc-containing decoy liposomes at 37 °C for 30 min, then used for intratracheal infection of C57BL/6 mice. Mice were monitored daily for survival. Mice that received LSTc decoy liposomes and PR/8 (dashed black line) had a 33% increase of mean survival time post infection as compared with mice that received control liposomes and PR/8 (solid black line) or PR/8 alone (gray dotted line). The LSTc decoy liposome and PR/8-treated mice survival advantage was statistically significant (χ² = 13.6, p ≤ 0.01; n ≥ 19 for each strain). Data shown are combined from three independent experiments, each having similar results.