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. 2013 Jan 30;288(12):8074–8084. doi: 10.1074/jbc.M112.432773

FIGURE 3.

FIGURE 3.

Increasing neuronal activity can overcome parvalbumin-dependent but not CaMBP4-dependent block of HDAC5 nuclear export. A, representative images of hippocampal neurons transfected with HDAC5 and with mCherry (mC.NLS) or CaMBP4-mCherry (CaMBP4-mC) as indicated. Neurons were either left untreated or treated with bicuculline. Scale bar is 10 μm. B, quantitative analysis of the subcellular localization of HDAC4 or HDAC5 in hippocampal neurons. Neurons were transfected with HDAC4 or HDAC5 and with mCherry or CaMBP4 and treated with bicuculline or not as indicated. Graphs show the ratio between cytoplasmic and nuclear localization. In total, 140–400 cells were analyzed for each condition from a minimum of five independent experiments. C, representative images of hippocampal neurons transfected with HDAC5 and with mCherry (mC.NLS) or parvalbumin-NLS-mCherry (PV.NLS-mC) as indicated. Neurons were either left untreated or treated with bicuculline. Scale bar is 10 μm. D, quantitative analysis of the subcellular localization of HDAC4 or HDAC5 in hippocampal neurons. Neurons were transfected with HDAC4 or HDAC5 and with mCherry or PV.NLS-mC and treated with bicuculline or not as indicated. Graphs show the ratio between cytoplasmic and nuclear localization. In total, 140–200 cells were analyzed for each condition from a minimum of five independent experiments. Statistically significant differences are indicated with asterisks (***, p < 0.001; *, p < 0.05, two-way ANOVA). Error bars, S.E.