FIGURE 5.

Shuttling of endogenous HDAC4 is responsive to nuclear calcium signaling. A, representative images showing the subcellular localization of endogenous HDAC4 in hippocampal neuron transfected with CaMBP4-mCherry (arrow) and nontransfected surrounding control cells. Scale bar is 20 μm. B, quantitative measurements of the relative fluorescent intensity of HDAC4 signal in the nucleus compared with the signal of the cytosol in untransfected (control) and CaMBP4-expressing cells. Each circle represents an independent experiment. Horizontal bars show mean values (control, 59.6 ± 3.6%; CaMBP4, 78.9 ± 5.9% p = 0.007 paired Student's t test). Statistically significant difference is indicated with asterisks (**). C, quantitative measurements of the relative fluorescent intensity of HDAC4 signal in the nucleus compared with the signal of the cytosol in mC.NLS- and CaMBP4-mC-expressing cells. Neurons were left untreated or treated with bicuculline and immunostained for endogenous HDAC4. The averaged results of three independent experiments are shown. Error bars, S.E.