Nuclear calcium controls HDAC4 and -5 nucleo-cytoplasmic shuttling in vivo.
A, immunohistochemistry of HDAC4 in the CA1 region of the hippocampus of adult mice stereotaxically injected with rAAV-mCherry-NLS (mC.NLS), rAAV-CAMBP4-mCherry (CaMBP4-mC) or with rAAV-parvalbumin.NLS-mCherry (PV.NLS-mC) using an anti-HDAC4 antibody, Hoechst to visualize the nuclei, and mCherry fluorescence to detect virus expression. B, immunohistochemistry of HDAC5 in the CA1 region of the hippocampus of adult mice stereotaxically injected with rAAV-mCherry-NLS (mC.NLS), rAAV-CAMBP4-mCherry (CaMBP4-mC), or with rAAV-parvalbumin.NLS-mCherry (PV.NLS-mC) using an anti-HDAC5 antibody, Hoechst to visualize the nuclei, and mCherry fluorescence to detect virus expression. C, immunohistochemistry of the CA1 region of the hippocampus of adult mice stereotaxically injected with rAAV-mCherry.NLS (mC.NLS), using a normal rabbit IgG antibody to test the specificity of the immunostainings shown in A and B, Hoechst to visualize the nuclei, and mCherry fluorescence to detect virus expression. Scale bar is 20 μm.