TABLE 4.
EptC is required for OM stability
Colony forming ability in the phosphate-limiting 121 medium at different concentrations of SDS or in the presence of 150 μm Zn2+. Cultures were adjusted to an A595 of 0.1, and viability was measured after plating 10-fold serial dilutions on agar plates containing 121 medium. Plates were incubated at 37 °C for 18 h.
| Strain | 0.25% SDS | 0.5% SDS | 1% SDS | 150 μm Zn2+ |
|---|---|---|---|---|
| Wild type | 3 × 107 | 3 × 107 | 2 × 107 | 3 × 107 |
| ΔeptA | 2 × 107 | 2 × 107 | 3 × 107 | 2 × 107 |
| ΔeptB | 2 × 107 | 3 × 107 | 2 × 107 | 3 × 107 |
| ΔeptC | 2 × 104 | 7 × 103 | 3 × 102 | 3 × 102 |
| Δ(eptB eptC) | 6 × 106 | 2 × 106 | 5 × 106 | 2 × 106 |
| ΔeptC + vector | 2 × 104 | 6 × 103 | 5 × 102 | 60 |
| ΔeptC + peptC+ | 3 × 107 | 3 × 107 | 3 × 107 | 3 × 107 |