FIGURE 5.

D. discoideum RNR mRNA and protein expression analyses. A, Northern blot analysis. Expression analysis of nrd genes and the mitochondrial large ribosomal RNA (rnlA) of vegetative cells (0 h) and during different stages of D. discoideum development is shown. The two lowest rows show ethidium bromide staining of the agarose gel prior to blotting from which the presence of the large and small rRNAs show equal loading of the RNA. B, reverse transcription-PCR of D. discoideum nrdJ with nrdA. Both nrdJ and nrdA transcripts are present in cDNA prepared from axenically growing cells (RT+ lanes), whereas no specific DNA fragment was amplified from the negative control template when reverse transcriptase was excluded (RT−). Gene-specific primers amplifying the full-length genes were used for comparison. C, Western blot analysis on protein extracts from different developmental stages.