FIGURE 7.

NEDD4L antagonizes Dvl2-induced axis duplication in Xenopus embryos. A, NEDD4L blocks axis duplication induced by wild-type Dvl2, but not by AY mutant. mRNA (1 ng) encoding wild-type or AY mutant human Dvl2 was injected alone or together with 500 pg of human NEDD4L mRNA into the ventral marginal zone at the 4-cell stage Xenopus embryos, and β-galactosidase mRNA (500 pg) was coinjected as a lineage marker. The phenotype was scored at the tadpole stage. B, quantitative analysis of the phenotype shown in A. The total number of injected embryos of each group was indicated.