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hCOA3 knockdown causes a defect in COX activity in HeLa cells. A, specific activity of the OXPHOS complexes. B, citrate synthase-specific activity. C, quantitative RT-PCR of hCOA3 mRNA. All experiments were performed in untransfected HeLa cells (untransfected), HeLa cells transfected with non-interfering siRNAs (Ki #2) or siRNAs against hCOA3 (siRNAs hCOA3) 96 h after transfection. Data represent mean values ± S.D. ***, p < 0.001 (n = 6).
