FIGURE 5.

Impact of Grx2^−/− on bioenergetics of mitochondria from liver (a) and skeletal muscle (b). Bioenergetic determinations were performed with the Seahorse XF24 Extracellular Flux Analyzer. Mitochondria (10 μg) were attached to the surface of Seahorse TC plates and then treated sequentially with ADP (0.1 mm), oligomycin (oligo; 2.5 μg/ml) and FCCP (8 μm) to test state 3, state 4, and maximal respiration, respectively. % contribution of respiration to ATP production was calculated by dividing state 4 respiration (proton leak-dependent) by state 3 respiration. Student's t test, n = 4, mean ± S.E. Pyr, pyruvate; Mal, malate.