TABLE 1.

Loss of Grx2 expression increases the rate of ROS production from liver but not skeletal muscle mitochondria

Rates of ROS production from mitochondria isolated from wild-type and Grx2 knock-out (Grx2^−/−) mice treated with or without DTT were calculated before and after the addition of oligomycin (Oligo). ROS production was measured using dihydrodichlorofluorescein diacetate. Arbitrary units/min/mg protein. Student's t test, n = 4, mean ± S.E. NA corresponds to no rate recorded.

	−DTT		+DTT
	WT	Grx2−/−	WT	Grx2−/−
Liver (−Oligo)	9.06 ± 5.86	42.63 ± 4.63a	NA	26.67 ± 7.71a
Liver (+Oligo)	52.11 ± 6.77	73.09 ± 14.41a	69.03 ± 7.37	79.57 ± 3.06
Muscle (−Oligo)	NA	NA	NA	NA
Muscle (+Oligo)	28.17 ± 4.51	32.33 ± 6.59	35.66 ± 3.97	37.08 ± 7.98

^a p < 0.05, statistical comparisons were performed between WT and Grx2^−/− mitochondria under each condition.