Δset1 has slightly higher frq and wc-1 expression under light-inducing conditions. Light-induced gene expression was examined in Δset1 (XB140-10) compared with WT (FGSC 2489), and relative levels of frq (A), wc-1 (B), and vvd (C) were determined by qRT-PCR. D, FRQ protein levels were also examined and appeared to be higher at later time points in Δset1 relative to WT. E, quantification of FRQ protein levels in response to light. Values are the normalized average of four independent experiments. F, level of WC-1 is generally lower in Δset1 compared with WT. All error bars represent the S.E.