FIGURE 7.

DNA methylation at frq requires SET1. A, methylation-sensitive Southern blot was performed on WT compared with Δset1 from DNA isolated from 12- and 24-h time points. DNA was digested with BfuCI (B) and DpnII (D) and resolved on a 1% agarose gel, and DNA methylation was determined by probing a region of the frq promoter that is methylated. A strain with the frq⁹ allele (hypomethylated) and a strain lacking the DNA methyltransferase dim-2 were added as controls. All the samples shown are contained on the same blot but separated to remove unrelated information. B, blot was stripped and probed a second time for a region within the mitochondrial genome. MitoP, mitochondrial probe. C, expression of the dim-2 transcript was analyzed by qRT-PCR in WT compared with Δset1.