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. 2013 Jan 14;288(12):8391–8404. doi: 10.1074/jbc.M112.408179

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Identification of Nes-S protein by immunoblotting of IF-enriched preparations of DRG with anti-AY14. A, dot blotting of the synthesized AY14 peptide with anti-AY14. The specificity of anti-AY14 was confirmed. WB, Western blotting. B, immunoblotting of IF-enriched preparations of DRG of adult rats with Rat-401 and anti-AY14. Although Rat-401 recognized the band corresponding to nestin-FL, anti-AY14 specifically recognized the 45.9-kDa band of Nes-S protein. Immunoblotting with anti-actin and immunoblotting with anti-NFH were used as loading controls.