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Filament assembly properties of Nes-S. A–I, double labeling of pEGFP-NestS-transfected SW13 cells with anti-GFP (A, D, and G) and anti-vimentin (B, E, and H). Nes-S presented a dispersed pattern in vimentin-free SW13 cells (A–C). The majority of the Nes-S protein co-localized with vimentin IF in the few SW13 cells that expressed vimentin (D–I). Scale bar: 15 μm.
