FIGURE 3.

Mutants with compromised pH homeostasis are hypersensitive to edelfosine. Strains scored as sensitive at least three times (from four screens completed) were verified by spotting serial dilutions of the cells onto media containing 19 μm edelfosine. Thus, a total of 54 deletion strains were confirmed to be edelfosine-sensitive. a, analysis was extended to all known V-ATPase-related genes by spotting serial dilutions of their corresponding deletion strains onto rich (YPD) medium control or plates containing 19 μm edelfosine (EDLF). Plates were incubated at 30 °C for 3 days. Data shown are representative of three independent experiments. b, deletion strains vma2, trk1, and snf1 were transformed with empty pRS315 (CEN) plasmids or the same plasmids containing the corresponding wild-type gene under their own promoters. Sensitivity to edelfosine was reverted to that displayed by the isogenic wild-type strain (BY4741) transformed with the empty plasmid. c, yeast strain with the PMA1-DAmP (decreased abundance by mRNA perturbation) allele displays increased sensitivity to edelfosine.