TABLE 2.
Complete list of genes identified in our genetic screen
The genes discussed in this work are in boldface italic.
| Sensitivity | Gene | Cellular role | Localization |
|---|---|---|---|
| +++ | AFG3 | Protein phosphorylation | Mitochondria |
| +++ | BEM1 | Cell organization and biogenesis | Bud tip-bud neck-cell periphery |
| +++ | DBF2 | Protein phosphorylation | Bud neck-cytoplasm |
| +++ | ERG3 | Lipid metabolism | ER |
| +++ | GAL11 | Transcription | Nucleus |
| +++ | HTA3 (HTZ1) | Chromatin remodeling | Nucleus |
| +++ | PGD1 | Transcription | Nucleus |
| +++ | REI1 | Translation | Cytoplasm-ribosome |
| +++ | SIN3 | Chromatin remodeling | Mitochondria-nucleus |
| +++ | SIP3 | Energy homeostasis | Nucleus |
| +++ | SNF1 | Energy homeostasis | Nucleus-mitochondria-vacuole-cytoplasm |
| +++ | SNF2 | Chromatin remodeling | Nucleus |
| +++ | SNF6 | Chromatin remodeling | Nucleus |
| +++ | SPT7 | Transcription | Nucleus-mitochondria |
| +++ | VMA16 (PPA1) | Vacuolar ATPase | Vacuole |
| +++ | VMA2 | Vacuolar ATPase | Vacuole |
| +++ | VMA4 | Vacuolar ATPase | Vacuole |
| +++ | VMA5 | Vacuolar ATPase | Vacuole |
| +++ | VMA9 | Vacuolar ATPase | Vacuole |
| +++ | VPH2 (VMA12) | Vacuolar ATPase assembly | ER |
| +++ | YJL175W | Chromatin remodeling | Probably SWI3 (nucleus) |
| ++ | AKR1 | Protein palmitoylation | Golgi |
| ++ | ASC1 | Translation | Cytosol-ribosome |
| ++ | BIM1 | Cell organization and biogenesis | Microtubule |
| ++ | COX17 | Cytochrome c assembly | Mitochondria-nucleus? |
| ++ | CYC2 | Cytochrome c regulation | Mitochondria |
| ++ | DEF1 | Protein degradation | Nucleus-cytoplasm |
| ++ | ELO2 | Lipid metabolism | ER |
| ++ | ETR1 | Lipid metabolism | Mitochondria-nucleus |
| ++ | LIP5 | Lipid metabolism | Mitochondria |
| ++ | NAP1 | Chromatin remodeling | Nucleus-cytoplasm |
| ++ | PAA1 | Chromatin remodeling | Cytoplasm |
| ++ | PDA1 | Acetyl-CoA production (impact on lipid metabolism) | mitochondria |
| ++ | RPB4 | Transcription | Nucleus |
| ++ | SEC22 | Vesicular transport | Golgi-ER |
| ++ | SER1 | Serine metabolism (impact on lipid metabolism) | Cytoplasm |
| ++ | SHY1 | Cytochrome c assembly | Mitochondria |
| ++ | SNF4 | Energy homeostasis | Nucleus-PM-cytoplasm |
| ++ | SNF5 | Chromatin remodeling | Nucleus |
| ++ | SPF1 | Ion transport (P-type ATPase) | ER-mitochondria |
| ++ | SPO14 | Lipid metabolism | Endosomes? |
| ++ | SRB2 | Transcription | Nucleus-cytoplasm |
| ++ | SUR4 | Lipid metabolism | ER |
| ++ | SWI6 | Transcription | Nucleus-cytoplasm |
| ++ | TRK1 | pH homeostasis and K+ transport | PM |
| ++ | UBP6 | Protein degradation | Nucleus-cytoplasm |
| ++ | VMA10 | Vacuolar ATPase | Vacuole |
| ++ | VMA11 (TFP3) | Vacuolar ATPase | Vacuole-ER |
| ++ | VMA21 | Vacuolar ATPase assembly | Vacuole |
| ++ | VMA7 | Vacuolar ATPase | Vacuole |
| ++ | YBR178W | Lipid metabolism | Probably EHT1 (mitochondria-lipid particle) |
| ++ | YDR521W | Transcription | Probably YDR520C (nucleus) |
| + | PDX3 | Lipid metabolism | ? |
| + | VMA6 | Vacuolar ATPase | Vacuole |