FIGURE 3.
The effects of alanine mutation in the luminal domain of SLN on the KCa and Vmax of SERCA. A, SDS-PAGE of co-reconstituted proteoliposomes. The top gel shows the incorporation of SERCA (2 μg of co-reconstituted proteoliposomes were loaded onto a 10% gel and stained with Coomassie). The bottom gel shows the incorporation of wild-type and mutant SLN (2 μg of co-reconstituted proteoliposomes were loaded onto a 16% gel and stained with silver). KCa (B) and Vmax (C) values were determined from ATPase activity measurements for SERCA in the absence and presence of wild-type and mutant forms of SLN. Each data point is the mean ± S.E. (error bars) (n ≥ 4). The Vmax, KCa, and nH are given in Table 1. Asterisks indicate comparisons against wild-type SLN (p < 0.05).