FIGURE 2.

Dose- and time-dependent effects of Bt₂cAMP on P-HSL, HSL, StAR, and P-StAR in MA-10 cells. Cells were treated in the absence or presence of Bt₂cAMP with either increasing doses (0.1–1.0 mm) for 6 h or a fixed dose (0.5 mm) for 12 h. Following treatments, cells were subjected to immunoblotting analyses (A and B). Representative immunoblots show P-HSL (Ser-660), HSL, StAR, and P-StAR using 20–30 μg of total cellular protein (A and B). Immunoblots shown are representative of four independent experiments. β-Actin expression was assessed as loading controls. Integrated optical density values of P-HSL, HSL, StAR, and P-StAR in each band were quantified and normalized with the corresponding β-actin expression, and compiled data are presented (n = 4, ± S.E.).