FIGURE 2.

DPP enhances osteoblastic gene expression in mesenchymal stem cells. C3H10T1/2 cells and HMSCs in basal media for 24 h were stimulated with 500 ng/ml rDPP with or without BAPTA-AM treatment for 4 and 24 h. Total RNA was isolated and subjected to real time PCR and analyzed for gene expression of Runx2, BSP, DMP1, Osterix, and OCN. Untreated C3H10T1/2 cells served as control. These results were normalized with the loading control GAPDH. Experiments were done in triplicate. *, #, (), and [caret], p < 0.05. C, control.