TABLE 4.

Rate constants for raloxifene (RAL) binding to SULT2A1 (WT and MT)

Enzyme	Enzyme species	kon	koff	Kd (koff/kon)
		m−1 s−1	s−1	μm
MT	E○a	3.5 (0.1)b × 105	0.43 (0.04)	1.2 (0.1)
	PAPS-E·	3.3 (0.2) × 105	0.50 (0.01)	1.5 (0.2)
WT	E○	4.1 (0.1) × 105	0.47 (0.03)	1.1 (0.1)
	PAPS-E·	2.2 (0.3) × 104	0.54 (0.03)	25 (5)

^a (·) indicates Raloxifene binding sites.

^b S.E. is given in parentheses.