FIGURE 4.

Autophagy does not influence the activation of caspase-1. Western blot analysis of caspase-1 in lysates of autophagy-impaired human PBMCs stimulated with B. burgdorferi (m.o.i., 2) was performed. PBMCs were preincubated for 1 h at 37 °C in RPMI 1640 medium alone, containing 10 mm 3MA or 100 nm wortmannin. After 3 h of stimulation with culture medium or B. burgdorferi, cells were lysed and further analyzed with Western blotting. The picture is representative for results obtained from four volunteers.