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HDX centroids shifts because of exosite ligand binding. Centroids for the ABE I segment 65–84 (A–D) and the ABE II segment 85–99 (E–H) following 1 min of deuteration in the following environments: free thrombin (A and E), Hirudin(54–65)-thrombin (B and F), PAR3(44–56)-thrombin (C and G), and PAR1(49–62)-thrombin (D and H).
