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Deuterium incorporation for the Hirudin(54–65)-thrombin complex in the presence and absence of PPACK. The bars in the graphs correspond to deuterium incorporation of free thrombin (white), Hirudin(54–65)-bound thrombin (white striped), PPACK-inhibited thrombin (gray), and PPACK-inhibited thrombin in the presence of Hirudin(54–65) (gray striped). Values for both 1 and 10 min of deuteration are displayed. The error bars correspond to standard deviations for three independent trials.
